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ETS2 Antibody

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Immunocytochemistry/ Immunofluorescence: ETS2 Antibody [NBP2-14951] - Paraformaldehyde-fixed HeLa, using ETS2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) ...read more
Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951] - Rat fore brain. ETS2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Chromatin Immunoprecipitation: ETS2 Antibody [NBP2-14951] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by ...read more
Immunoprecipitation: ETS2 Antibody [NBP2-14951] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 ug of ...read more
Western Blot: ETS2 Antibody [NBP2-14951] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Biological Strategies: Western Blot: ETS2 Antibody [NBP2-14951] -Untreated (–) and treated (+) Raw 264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 ...read more
Western Blot: ETS2 Antibody [NBP2-14951] - Untreated (-) and treated (+) Raw 264.7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody (NBP2-14951) diluted at ...read more
Western Blot: ETS2 Antibody [NBP2-14951] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody (NBP2-14951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

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ETS2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETS2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for ETS2 Antibody

  • ETS2IT1
  • human erythroblastosis virus oncogene homolog 210protein C-ets-2
  • oncogene ETS-2
  • v-ets avian erythroblastosis virus E2 oncogene homolog 2
  • v-ets avian erythroblastosis virus E26 oncogene homolog 2
  • v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Background

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETS2
Uniprot