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ErbB4/Her4 Antibody (HFR1) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
HFR1
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2662AF350
Alexa Fluor 405
NB100-2662AF405
Alexa Fluor 488
NB100-2662AF488
Alexa Fluor 532
NB100-2662AF532
Alexa Fluor 594
NB100-2662AF594
Alexa Fluor 647
NB100-2662AF647
Alexa Fluor 700
NB100-2662AF700
Alexa Fluor 750
NB100-2662AF750
Allophycocyanin/Cy7
NB100-2662APCCY7
Biotin
NB100-2662B
DyLight 350
NB100-2662UV
DyLight 405
NB100-2662V
DyLight 488
NB100-2662G
DyLight 550
NB100-2662R
DyLight 594
NB100-2662DL594
DyLight 650
NB100-2662C
DyLight 680
NB100-2662FR
DyLight 755
NB100-2662IR
FITC
NB100-2662F
HRP
NB100-2662H
Janelia Fluor 525
NB100-2662JF525
Janelia Fluor 549
NB100-2662JF549
Janelia Fluor 646
NB100-2662JF646
Janelia Fluor 669
NB100-2662JF669
PE/Atto594
NB100-2662PEATT594
PE/Cy5.5
NB100-2662PECY55
PE/Cy7
NB100-2662PECY7
Unconjugated
NB100-2662
mFluor Violet 450 SE
NB100-2662MFV450
mFluor Violet 500 SE
NB100-2662MFV500
mFluor Violet 610 SE
NB100-2662MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-2662
BSA Free
NB100-2662
BSA Free
NB100-2662-0.025mg
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ErbB4/Her4 Antibody (HFR1) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein (residues 1249-1264.)
Localization
Type I membrane protein
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ERBB4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ErbB4/Her4 Antibody (HFR1) [CoraFluor™ 1]

  • avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB4
  • HER4
  • HER4MGC138404
  • p180erbB4
  • Proto-oncogene-like protein c-ErbB-4
  • receptor tyrosine-protein kinase erbB-4
  • Tyrosine kinase-type cell surface receptor HER4
  • v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ErbB4/Her4
Antibody (HFR1) [Cora ...
NB100-2662CL1
ErbB4/Her4 Antibody (HFR1) [CoraFluor™ 1]
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ERBB4