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NEU-1/Sialidase-1 Antibody (688215)

 (2 Publications)
 (1 Review)
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
688215
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB6860-SP
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NEU-1/Sialidase-1 Antibody (688215) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
Lys46-Leu415
Accession # Q99519
Specificity
Detects human NEU-1/Sialidase-1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NEU1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB6860 in the following applications:
Publications
Read Publications using
MAB6860 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEU-1/Sialidase-1 Antibody (688215)

  • Acetylneuraminyl hydrolase
  • EC 3.2.1.18
  • exo-alpha-sialidase
  • FLJ93471
  • G9 sialidase
  • Lysosomal sialidase
  • N-acetyl-alpha-neuraminidase 1
  • NANH
  • NEU
  • NEU1
  • NEU-1
  • SIAL1
  • sialidase 1 (lysosomal sialidase)
  • Sialidase-1

Background

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NEU-1/Sialidase-1 Antibody (MAB6860)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Review for NEU-1/Sialidase-1 Antibody (MAB6860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6860:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Human 02/25/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 24200502
SpeciesHuman

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Recent Reviews

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 Verified Customer
02/25/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol NEU1
Uniprot