Home » ErbB2/Her2 » ErbB2/Her2 Antibodies » ErbB2/Her2 Antibody (191924) [Alexa Fluor® 594]
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ErbB2/Her2 Antibody (191924) [Alexa Fluor® 594]

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MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB1129T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). View our protocol for Staining Membrane-associated Proteins.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB1129T, filled histogram) or isotype control antibody (Catalog # ...read more
MDA-MB-453 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB1129T, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
191924
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1129U-100UG
Alexa Fluor 405
FAB1129V-100UG
Alexa Fluor 488
FAB1129G-025
Alexa Fluor 532
FAB1129X
Alexa Fluor 647
FAB1129R-025
Alexa Fluor 700
FAB1129N-100UG
Alexa Fluor 750
FAB1129S-100UG
Allophycocyanin
FAB1129A
Allophycocyanin/Cy7
FAB1129APCCY7
Biotin
FAB1129B
DyLight 350
FAB1129D
DyLight 405
FAB1129E
DyLight 488
FAB1129K
DyLight 550
FAB1129L
DyLight 594
FAB1129M
DyLight 650
FAB1129W
DyLight 680
FAB1129Y
DyLight 755
FAB1129Z
FITC
FAB1129F
HRP
FAB1129H
Janelia Fluor 549
FAB1129I
Janelia Fluor 646
FAB1129J
PE/Atto594
FAB1129PEATT594
PE/Cy5.5
FAB1129PECY55
PE/Cy7
FAB1129PECY7
PerCP
FAB1129C
Phycoerythrin
FAB1129P
Unconjugated
MAB1129-100
mFluor Violet 450 SE
FAB1129MFV450
mFluor Violet 500 SE
FAB1129MFV500
mFluor Violet 610 SE
FAB1129MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1129-100
Carrier Free
FAB1129A
Carrier Free
FAB1129P
Carrier Free
MAB1129-SP
Carrier Free
MAB1129-100
Carrier Free
MAB1129-500
Carrier Free
FAB1129R-025
Carrier Free
FAB1129G-025
Carrier Free
FAB1129V-100UG
Carrier Free
FAB1129U-100UG
Carrier Free
FAB1129S-100UG
Carrier Free
FAB1129N-100UG
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ErbB2/Her2 Antibody (191924) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626
Specificity
Detects human ErbB2/Her2 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ERBB2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
Reviewed Applications
Read 1 Review rated 5
using
FAB1129T in the following application:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (191924) [Alexa Fluor® 594]

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (Human Epidermal Growth Factor Receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-Kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-Catenin and Plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for ErbB2/Her2 Antibody (FAB1129T) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using FAB1129T:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Jan Martinek
 IF Human 06/17/2021
View

Summary

ApplicationImmunofluorescence
Sample TestedHEK 293 cell line
SpeciesHuman
LotAEVJ0120091

Comments

Comments4% PFA fixed cells , Triton X-100 permeabilization
***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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FAQs for ErbB2/Her2 Antibody (FAB1129T). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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Immunofluorescence ErbB2/Her2 FAB1129T
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 Jan Martinek
06/17/2021
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol ERBB2
Uniprot