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ErbB2/Her2 Antibodies

We offer ErbB2/Her2 Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Inhibition of Cell Growth, Sandwich ELISA, Simple Western, Western Blot. Each ErbB2/Her2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ErbB2/Her2 Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Rat. Use the list below to choose the ErbB2/Her2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ErbB2/Her2 polyclonal antibodies and browse our ErbB2/Her2 monoclonal antibody catalog.

Alternate Names for ErbB2/Her2 Antibodies

anti-ErbB2/Her2 antibody, anti-ERBB2 antibody, anti-c-erb B2/neu protein antibody, anti-CD340 antibody, anti-CD340 antigen antibody, anti-EC 2.7.10 antibody, anti-HER-2 antibody, anti-HER2EC 2.7.10.1 antibody, anti-herstatin antibody, anti-Metastatic lymph node gene 19 protein antibody, anti-MLN 19 antibody, anti-MLN19 antibody, anti-NEUHER-2/neu antibody, anti-neuroblastoma/glioblastoma derived oncogene homolog antibody, anti-NGLTKR1 antibody, anti-p185erbB2 antibody, anti-Proto-oncogene c-ErbB-2 antibody, anti-Proto-oncogene Neu antibody, anti-receptor tyrosine-protein kinase erbB-2 antibody, anti-Tyrosine kinase-type cell surface receptor HER2 antibody, anti-v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog) antibody, anti-v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) antibody

77 Results for "erbb2-her2" in Primary Antibodies

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AF1129

ErbB2/Her2 Antibody [Unconjugated]

(3 Reviews)

(18 Publications)

100 μg

AF1129

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1129

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1129-SP

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MAB1129

ErbB2/Her2 Antibody (191924) [Unconjugated]

(15 Publications)

100 μg

MAB1129

ErbB2/Her2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane/cytoplasm in glans and villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1129U-100UG
Alexa Fluor 405	FAB1129V-100UG
Alexa Fluor 488	FAB1129G-025
Alexa Fluor 532	FAB1129X
Alexa Fluor 594	FAB1129T-025
Alexa Fluor 647	FAB1129R-025
Alexa Fluor 700	FAB1129N-100UG
Alexa Fluor 750	FAB1129S-100UG
Allophycocyanin	FAB1129A
Allophycocyanin/Cy7	FAB1129APCCY7
Biotin	FAB1129B
DyLight 350	FAB1129D
DyLight 405	FAB1129E
DyLight 488	FAB1129K
DyLight 550	FAB1129L
DyLight 594	FAB1129M
DyLight 650	FAB1129W
DyLight 680	FAB1129Y
DyLight 755	FAB1129Z
FITC	FAB1129F
HRP	FAB1129H
Janelia Fluor 549	FAB1129I
Janelia Fluor 646	FAB1129J
PE/Atto594	FAB1129PEATT594
PE/Cy5.5	FAB1129PECY55
PE/Cy7	FAB1129PECY7
PerCP	FAB1129C
Phycoerythrin	FAB1129P
mFluor Violet 450 SE	FAB1129MFV450
mFluor Violet 500 SE	FAB1129MFV500
mFluor Violet 610 SE	FAB1129MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1129A
Carrier Free	FAB1129P
Carrier Free	MAB1129-SP
Carrier Free	MAB1129-500
Carrier Free	FAB1129R-025
Carrier Free	FAB1129G-025
Carrier Free	FAB1129T-025
Carrier Free	FAB1129V-100UG
Carrier Free	FAB1129U-100UG
Carrier Free	FAB1129S-100UG
Carrier Free	FAB1129N-100UG

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MAB4360

ErbB2/Her2 Antibody (416727)

(1 Review)

(11 Publications)

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4360-SP

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AF1768

ErbB2/Her2 [p Tyr1248] Antibody [Unconjugated]

(8 Publications)

50 μg

AF1768

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes or with 10 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-ErbB2/Her2 (Y1248) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1768) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ErbB2/Her2 (Y1248) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-ErbB2/Her2 (Y1248) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1768) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ErbB2/Her2 (Y1248) at approximately 265 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-ErbB2/Her2 (Y1248) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1768). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Antitumor effects of lapatinib and PD168393 in the ErbB2 positive lung cancer cell line, Calu3 and the ErbB2 positive breast cancer cell line, SkBr3 after 72 hour treatment.A. Cellular proliferation of Calu3 and SkBr3 cells treated with different concentrations of lapatinib and PD168393 as determined by the MTS assay. B. Lapatinib and PD168393 induce cellular apoptosis in Calu3 and SkBr3 cells. Representative Annexin V/propidium iodide flow cytometry; the numbers represent percentage of cells in the appropriate quadrant. C. Quantification of apoptosis. D. Lapatinib induces cell cycle arrest in Calu3 cells as detected by BrdU and 7-ADD assay. R1-G1 phase; R2-G2 phase; R3-S phase; R4-G0 phase. E. Dose response of lapatinib and PD168393 on phosphorylation of ErbB2, AKT and ERK in Calu3 and SkBr3 cells in response to EGF treatment. p-ErbB2: phosphorylated ErbB2; t-ErbB2: total-ErbB2; p-AKT: phosphorylated AKT; t-AKT: total AKT; p-ERK: phosphorylated ERK; t-ERK: total ERK. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106349), licensed under a CC-BY license. Not internally tested by R&D Systems.

Downregulation of PHLDA1 expression upon ErbB2/EGFR inhibition.Western blot analysis following treatment with lapatinib, erlotinib and CL387,785 at 1 µm for 6, 24 and 48 hours in ErbB2-positive SkBr3 and EGFR-mutated H1975, HCC827. DMSO treatment was 48 hours. Different cell lines were used with the relevant inhibitor capable of blockade of oncogenic signaling. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106349), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1768-SP

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MAB9589

ErbB2/Her2 Antibody (Hu5) [Unconjugated]

(1 Review)

(4 Publications)

100 μg

MAB9589

MDA-MB-453 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589, filled histogram) or Human IgG Control Antibody (1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589) inhibits proliferation in the SK‑BR‑3 human breast cancer cell line as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 15‑75 ng/mL.

ADCC assays with SKOV-3 and Raji targets. NK cells were cultured as described in the adjacent figure for 13 days. Prior to setting up the ADCC assay, CD56+CD16+ levels were ascertained to be >30% on NKs using Human Fc gamma RIII (CD16) PerCP-conjugated Antibody (FAB2546C); in our hands, this is the minimum level required for killing via ADCC. Target cells were labeled with Mito Mark Green (SKOV-3 or Raji), and Effectors/NKs were labeled with Janelia Fluor 646 as described (Figure 1). (A) SKOV-3 cells were preincubated with 5 ug/mL Anti-Her2/Trastuzumab (Catalog # MAB9589); (B) Raji cells were preincubated with 10 ug/mL Anti-CD20/Rituximab (MAB9630), both for 30 minutes at room temperature prior to setting up the ADCC assay with the NKs. 0.5:1 E:T ratios were used for the SKOV-3/Trastuzumab model, and NKs and SKOV-3 cells were co-incubated for 2 hours at 37°C. 2:1 E:T ratios were used for the Raji/Rituximab model, and NKs and Raji cells were co-incubated for 2.5 hours at 37°C. In both models, the cells were harvested, stained with antibodies for Human CD3 Alexa Fluor 405 and Human CD56 Alexa Fluor 700, and analyzed by flow cytometry. Importantly, non-NKs (JF 646-CD56- cells) are shown in the Mito Mark dye graphs that depict killing. These results are representative of 3+ experiments.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Human Monoclonal

Host: Human Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9589U-100UG
Alexa Fluor 405	FAB9589V-100UG
Alexa Fluor 488	FAB9589G-100UG
Alexa Fluor 532	FAB9589X
Alexa Fluor 594	FAB9589T-100UG
Alexa Fluor 647	FAB9589R-100UG
Alexa Fluor 700	FAB9589N-100UG
Alexa Fluor 750	FAB9589S-100UG
Allophycocyanin	FAB9589A
Allophycocyanin/Cy7	FAB9589APCCY7
Biotin	FAB9589B-100
CoraFluor 1	FAB9589CL1
DyLight 350	FAB9589D
DyLight 405	FAB9589E
DyLight 488	FAB9589K
DyLight 550	FAB9589L
DyLight 594	FAB9589M
DyLight 650	FAB9589W
DyLight 680	FAB9589Y
DyLight 755	FAB9589Z
FITC	FAB9589F
HRP	FAB9589H
Janelia Fluor 525	FAB9589JF525
Janelia Fluor 549	FAB9589I
Janelia Fluor 646	FAB9589J
Janelia Fluor 669	FAB9589JF669
PE/Atto594	FAB9589PEATT594
PE/Cy5.5	FAB9589PECY55
PE/Cy7	FAB9589PECY7
PerCP	FAB9589C
Phycoerythrin	FAB9589P-100
mFluor Violet 450 SE	FAB9589MFV450
mFluor Violet 500 SE	FAB9589MFV500
mFluor Violet 610 SE	FAB9589MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB9589B-100
Carrier Free	MAB9589-SP
Carrier Free	FAB9589V-100UG
Carrier Free	FAB9589T-100UG
Carrier Free	FAB9589R-100UG
Carrier Free	FAB9589S-100UG
Carrier Free	FAB9589N-100UG
Carrier Free	FAB9589U-100UG
Carrier Free	FAB9589G-100UG
Carrier Free	FAB9589P-100

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NBP2-29624

ErbB2/Her2 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP2-29624

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-29624SS

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NB100-81960

ErbB2/Her2 [p Tyr1248] Antibody

(3 Publications)

0.05 ml

NB100-81960

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Staining of methanol-fixed MCF7 cells using HER2 phospho-antibody.

Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Human lung carcinoma tissue.

Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NB100-81960. The picture on the right is blocked with the phospho peptide.

<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using NB100-81960. The lane on the right is blocked with the phospho peptide.

Western Blot: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Western Blot analysis of 293 cells using NB100-81960 diluted at 1: 1000

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1248] Antibody [NB100-81960] - Mutant ERBB2 cooperates with loss of p53 & leads to papillary GBC in recipient mice. (A) Top: Schematic of human ERBB2, indicating the location of two point mutants (S310F & V777L). Bottom: retroviral vector used to transduce organoids, that had been treated with an sgp53-containing plasmid (px459) to induce loss of p53. (B) Immunofluorescence for ERBB2 (top) & phospho-ERBB2 (bottom) on organoids harboring the indicated genetic alterations. (C) Tumor volumes 36 days after s.c. implantation of the respective organoids into recipient mice. All mice transplanted with sgp53;ERBB2S310F- & sgp53;ERBB2V777L organoids exhibited tumor development, whereas sgp53;empty vector- & sgp53;ERBB2wildtype organoids did not give rise to tumors over a four-month observation period. There was no significant difference in the tumor burden of mice transplanted with sgp53;ERBB2S310F- & sgp53;ERBB2V777L organoids (p = 0.999). (D) Mice transplanted with sgp53;ERBB2S310F- & sgp53;ERBB2V777L organoids reached endpoint criteria with a median survival of 79.5 days & 58.5 days, respectively. (E) H&E & IHC for CK19 & EGFP on tumors generated with sgp53;ERBB2S310- & sgp53;ERBB2V777L organoids. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795490), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-90189

Alt Catalog# IMG-90189

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-01152

ErbB2/Her2 Antibody (OTI4F10)

(1 Publication)

0.1 ml

NBP2-01152

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.

Flow Cytometry: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.

Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.

Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70653AF350
Alexa Fluor 405	NBP2-70653AF405
Alexa Fluor 488	NBP2-70653AF488
Alexa Fluor 532	NBP2-70653AF532
Alexa Fluor 594	NBP2-70653AF594
Alexa Fluor 647	NBP2-70653AF647
Alexa Fluor 700	NBP2-70653AF700
Alexa Fluor 750	NBP2-70653AF750
Allophycocyanin	NBP2-70653APC
Allophycocyanin/Cy7	NBP2-70653APCCY7
Biotin	NBP2-70653B
CoraFluor 1	NBP2-70653CL1
DyLight 350	NBP2-70653UV
DyLight 405	NBP2-70653V
DyLight 488	NBP2-70653G
DyLight 550	NBP2-70653R
DyLight 594	NBP2-70653DL594
DyLight 650	NBP2-70653C
DyLight 680	NBP2-70653FR
DyLight 755	NBP2-70653IR
FITC	NBP2-70653F
HRP	NBP2-70653H
Janelia Fluor 525	NBP2-70653JF525
Janelia Fluor 549	NBP2-70653JF549
Janelia Fluor 646	NBP2-70653JF646
Janelia Fluor 669	NBP2-70653JF669
PE	NBP2-70653PE
PE/Atto594	NBP2-70653PEATT594
PE/Cy5.5	NBP2-70653PECY55
PE/Cy7	NBP2-70653PECY7
PerCP	NBP2-70653PCP
mFluor Violet 450 SE	NBP2-70653MFV450
mFluor Violet 500 SE	NBP2-70653MFV500
mFluor Violet 610 SE	NBP2-70653MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70653

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MAB11291

ErbB2/Her2 Antibody (416711) [Unconjugated]

(1 Review)

100 μg

MAB11291

HER2 was detected in immersion fixed paraffin-embedded sections of human breast cancer using Mouse Anti-Human ErbB2/HER2 Monoclonal Antibody (Catalog # MAB11291) at 12ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11291-SP

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NBP1-84584

ErbB2/Her2 Antibody

(3 Publications)

0.1 ml

NBP1-84584

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows no positivity in tumor cells as expected.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human kidney shows weak to moderate granular cytoplasmic positivity in cells in tubules.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF5176

ErbB2/Her2 Antibody

(4 Publications)

100 μg

AF5176

ErbB2/Her2 was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5176) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5176-SP

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NBP2-33064

ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free

0.1 mg

NBP2-33064

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Protein Array: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33064AF350
Alexa Fluor 405	NBP2-33064AF405
Alexa Fluor 488	NBP2-33064AF488
Alexa Fluor 532	NBP2-33064AF532
Alexa Fluor 594	NBP2-33064AF594
Alexa Fluor 647	NBP2-33064AF647
Alexa Fluor 700	NBP2-33064AF700
Alexa Fluor 750	NBP2-33064AF750
Allophycocyanin	NBP2-33064APC
Allophycocyanin/Cy7	NBP2-33064APCCY7
Biotin	NBP2-33064B
DyLight 350	NBP2-33064UV
DyLight 405	NBP2-33064V
DyLight 488	NBP2-33064G
DyLight 550	NBP2-33064R
DyLight 594	NBP2-33064DL594
DyLight 650	NBP2-33064C
DyLight 680	NBP2-33064FR
DyLight 755	NBP2-33064IR
FITC	NBP2-33064F
HRP	NBP2-33064H
Janelia Fluor 549	NBP2-33064JF549
Janelia Fluor 646	NBP2-33064JF646
PE	NBP2-33064PE
PE/Atto594	NBP2-33064PEATT594
PE/Cy5.5	NBP2-33064PECY55
PE/Cy7	NBP2-33064PECY7
mFluor Violet 450 SE	NBP2-33064MFV450
mFluor Violet 500 SE	NBP2-33064MFV500
mFluor Violet 610 SE	NBP2-33064MFV610

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

ErbB2/Her2 Antibodies

ErbB2/Her2 Antibody [Unconjugated]

ErbB2/Her2 Antibody (191924) [Unconjugated]

ErbB2/Her2 Antibody (416727)

ErbB2/Her2 [p Tyr1248] Antibody [Unconjugated]

ErbB2/Her2 Antibody (Hu5) [Unconjugated]

ErbB2/Her2 Antibody - BSA Free

ErbB2/Her2 [p Tyr1248] Antibody

ErbB2/Her2 Antibody (OTI4F10)

ErbB2/Her2 Antibody (416711) [Unconjugated]

ErbB2/Her2 Antibody

ErbB2/Her2 Antibody

ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free