Ephrin-B3 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 aa long and are highly conserved, especially the last 33 aa. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B3 has been shown to bind EphA4, EphB1, EphB2, and EphB3 (2, 3). The extracellular domains of murine and human Ephrin-B3 share 98% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.

Showing Publications 1 - 5 of 5.

Publications using AF395	Applications	Species
Abdul Shukkur Ebrahim, Zeyad Hailat, Sudeshna Bandyopadhyay, Daniel Neill, Mustapha Kandouz The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast Cancer International Journal of Molecular Sciences 2021-07-28 [PMID: 34360867]
Jeremiah P. C. Stinson, Cooper A. Brett, Julianne B. Carroll, Mark L. Gabriele Registry of Compartmental Ephrin-B3 Guidance Patterns With Respect to Emerging Multimodal Midbrain Maps Frontiers in Neuroanatomy 2021-03-16 [PMID: 33815071]
Beatriz Garcia-Diaz, Corinne Bachelin, Fanny Coulpier, Gaspard Gerschenfeld, Cyrille Deboux, Violetta Zujovic et al. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling Acta Neuropathologica 2019-09-01 [PMID: 31011859]
Kayser MS, McClelland AC, Hughes EG, Dalva MB Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors. J. Neurosci., 2006-11-22;26(47):12152-64. 2006-11-22 [PMID: 17122040] (ICC, Rat)	ICC	Rat
Yang NY, Pasquale EB, Owen LB, Ethell IM The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 2006-08-31;281(43):32574-86. 2006-08-31 [PMID: 16950769] (Flow Cytometry, Western Blot, Mouse)	Flow Cytometry, Western Blot	Mouse

Array AF395

• Ephrin-B3 Antibodies
• Ephrin-B3 Protein
• Ephrin-B3 Proteins and Enzymes