EphB3 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6‑9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10‑12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10‑12, 15).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow Cytometry, IF, IHC, IHC-Fr, IHC-P, Western Blot.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using AF432	Applications	Species
M Azkanaz, B Corominas-, SIJ Ellenbroek, L Bruens, AT Webb, D Laskaris, KC Oost, SJA Lafirenze, K Annusver, HA Messal, S Iqbal, DJ Flanagan, DJ Huels, F Rojas-Rodr, M Vizoso, M Kasper, OJ Sansom, HJ Snippert, P Liberali, BD Simons, P Katajisto, E Hannezo, J van Rheene Retrograde movements determine effective stem cell numbers in the intestine Nature, 2022-07-13;0(0):. 7/13/2022 [PMID: 35831497]
AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse)	IF	Mouse
L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):. 2020-01-28 [PMID: 32004754] (IHC, Mouse)	IHC	Mouse
AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (IHC, Mouse)	IHC	Mouse
Relation T, Yi T, Guess AJ et al. Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation Cell Stem Cell 5/18/2016 [PMID: 27184401]
Omanma Adighibe, Russell D. Leek, Marta Fernandez-Mercado, Jiangting Hu, Cameron Snell, Kevin C. Gatter et al. Why some tumours trigger neovascularisation and others don’t: the story thus far Chinese Journal of Cancer 2016-12-01 [PMID: 26873439]
Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. PLoS Genet, 2015-11-03;11(11):e1005638. 2015-11-03 [PMID: 26528816] (IHC-P, Mouse)	IHC-P	Mouse
Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells World Journal of Gastroenterology 2015-01-14 [PMID: 25593460]
Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6. 2011-01-26 [PMID: 21276420] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
Yoshitatsu Sei, Xinping Lu, Alice Liou, Xilin Zhao, Stephen A. Wank A stem cell marker-expressing subset of enteroendocrine cells resides at the crypt base in the small intestine American Journal of Physiology-Gastrointestinal and Liver Physiology 2011-02-01 [PMID: 21088235]
Bush JO, Soriano P Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev., 2009-06-10;23(13):1586-99. 2009-06-10 [PMID: 19515977] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Yang NY, Pasquale EB, Owen LB, Ethell IM The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 2006-08-31;281(43):32574-86. 2006-08-31 [PMID: 16950769] (Flow Cytometry, Western Blot, Mouse)	Flow Cytometry, Western Blot	Mouse
Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J EphB receptors coordinate migration and proliferation in the intestinal stem cell niche. Cell, 2006-06-16;125(6):1151-63. 2006-06-16 [PMID: 16777604] (IHC, Mouse)	IHC	Mouse
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006-02-01;119(0):559-70. 2006-02-01 [PMID: 16443753] (IHC, Mouse)	IHC	Mouse
Show All 15 Publications. Collapse Publications.

Array AF432

• EphB3 Antibodies
• EphB3 Lysate
• EphB3 Proteins
• EphB3 Proteins and Enzyme