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Endothelin-1 Antibody

Images

 
Western Blot: Endothelin-1 Antibody [NBP1-84779] - Analysis in human plasma.
Immunocytochemistry/ Immunofluorescence: Endothelin-1 Antibody [NBP1-84779] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & the Golgi apparatus. Antibody staining is shown in green
Immunohistochemistry-Paraffin: Endothelin-1 Antibody [NBP1-84779] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells and endothelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Endothelin-1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EDN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Endothelin-1 Recombinant Protein Antigen (NBP1-84779PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Endothelin-1 Antibody

  • ARCND3
  • EDN1
  • endothelin 1
  • Endothelin-1
  • ET1
  • HDLCQ7
  • PPET1
  • preproendothelin-1
  • QME

Background

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EDN1