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EEFSEC Antibody

Images

 
Western Blot: EEFSEC Antibody [NBP1-83992] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-173
Immunocytochemistry/ Immunofluorescence: EEFSEC Antibody [NBP1-83992] - Staining of human cell line A-431 shows localization to nucleus, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: EEFSEC Antibody [NBP1-83992] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EEFSEC Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEFSEC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EEFSEC Protein (NBP1-83992PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EEFSEC Antibody

  • eukaryotic elongation factor, selenocysteine-tRNA-specific

Background

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replacesEF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EEFSEC