![Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0004.jpg "Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary. |
| Localization | Cytoplasm, Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EEF1E1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for EEF1E1 Antibody (NBP1-32184)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EEF1E1 |
![Immunocytochemistry/Immunofluorescence: EEF1E1 Antibody [NBP1-32184] - Analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32184-img0005.jpg "Immunocytochemistry/Immunofluorescence: EEF1E1 Antibody [NBP1-32184] - Analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunohistochemistry-Paraffin-NBP1-32184-img0007.jpg "Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0003.jpg "Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunohistochemistry-Paraffin-NBP1-32184-img0006.jpg "Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Knockout Validated: EEF1E1 Antibody [NBP1-32184] - Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0008.jpg "Knockout Validated: EEF1E1 Antibody [NBP1-32184] - Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")