Novus Biologicals products are now on bio-techne.com

EAAT4 Overexpression Lysate

Images

 
Western Blot: EAAT4 Overexpression Lysate (Adult Normal) [NBP2-10518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EAAT4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

EAAT4 Overexpression Lysate Summary

Description

EAAT4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC221087

Accession#: NM_005071

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SLC1A6

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
61.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for EAAT4 Overexpression Lysate

  • EAAT4
  • EAAT4MGC43671
  • excitatory amino acid transporter 4
  • MGC33092
  • SLC1A6
  • Sodium-dependent glutamate/aspartate transporter
  • solute carrier family 1 (high affinity aspartate/glutamate transporter), member6
  • Solute carrier family 1 member 6

Background

SLC1A6 - solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1869
Species: Hu, Mu, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-59319
Species: Hu, Rt
Applications: ICC/IF, IP, WB
NBP1-20136
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, In vivo, ISH, WB
NBP2-81802
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP1-76578
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-54439
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, PA, WB
NLS799
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB110-39033
Species: Ch, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP3-13165
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
314-BP
Species: Hu
Applications: BA, BA
NBP1-46328
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-50028
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-P, WB
NLS3208
Species: Bv, Eq, Hu, Pm, Po, Pm
Applications: ICC, IHC, IHC-P, WB
NBP1-83314
Species: Ch, Hu
Applications: IHC, IHC-P, WB
NBP2-10518
Species: Hu
Applications: WB

Publications for EAAT4 Lysate (NBP2-10518) (0)

There are no publications for EAAT4 Lysate (NBP2-10518).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EAAT4 Lysate (NBP2-10518) (0)

There are no reviews for EAAT4 Lysate (NBP2-10518). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EAAT4 Lysate (NBP2-10518) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EAAT4 Products

Research Areas for EAAT4 Lysate (NBP2-10518)

Find related products by research area.

Blogs on EAAT4

There are no specific blogs for EAAT4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

GFAP Antibody
NB300-141

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EAAT4 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC1A6