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E-Selectin/CD62E Antibody (P2H3) - BSA Free

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Flow Cytometry: E-selectin Antibody (P2H3) [NBP1-43393] - Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD62E (E- Selectin) Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE . Total viable cells were used for analysis.
Flow Cytometry: E-selectin Antibody (P2H3) [NBP1-43393] - Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IP
Clone
P2H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43393AF350
Alexa Fluor 405
NBP1-43393AF405
Alexa Fluor 488
NBP1-43393AF488
Alexa Fluor 532
NBP1-43393AF532
Alexa Fluor 594
NBP1-43393AF594
Alexa Fluor 647
NBP1-43393AF647
Alexa Fluor 700
NBP1-43393AF700
Alexa Fluor 750
NBP1-43393AF750
Allophycocyanin
NBP1-43393APC
Allophycocyanin/Cy7
NBP1-43393APCCY7
Biotin
NBP1-43393B
CoraFluor 1
NBP1-43393CL1
DyLight 350
NBP1-43393UV
DyLight 405
NBP1-43393V
DyLight 488
NBP1-43393G
DyLight 550
NBP1-43393R
DyLight 594
NBP1-43393DL594
DyLight 650
NBP1-43393C
DyLight 680
NBP1-43393FR
DyLight 755
NBP1-43393IR
FITC
NBP1-43393F
HRP
NBP1-43393H
Janelia Fluor 525
NBP1-43393JF525
Janelia Fluor 549
NBP1-43393JF549
Janelia Fluor 646
NBP1-43393JF646
Janelia Fluor 669
NBP1-43393JF669
PE
NBP1-43393PE
PE/Atto594
NBP1-43393PEATT594
PE/Cy5.5
NBP1-43393PECY55
PE/Cy7
NBP1-43393PECY7
PerCP
NBP1-43393PCP
mFluor Violet 450 SE
NBP1-43393MFV450
mFluor Violet 500 SE
NBP1-43393MFV500
mFluor Violet 610 SE
NBP1-43393MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-43393-0.025mg
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E-Selectin/CD62E Antibody (P2H3) - BSA Free Summary

Immunogen
The immunogen for this antibody was CD62E.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELE
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for E-Selectin/CD62E Antibody (P2H3) - BSA Free

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium. The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SELE