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DNAM-1/CD226 Antibody (102511) [Unconjugated]

 (10 Publications)
 (1 Review)
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Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human ...read more
In a functional ELISA, Human DNAM-1/CD226 Antibody (Catalog # MAB666) blocks the binding of Recombinant Human DNAM-1/CD226 Fc Chimera (666-DN) to Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (AVI9174). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
102511
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB666U-100UG
Alexa Fluor 405
FAB666V-100UG
Alexa Fluor 488
FAB666G-100UG
Alexa Fluor 532
FAB666X
Alexa Fluor 594
FAB666T-100UG
Alexa Fluor 647
FAB666R-100UG
Alexa Fluor 700
FAB666N-100UG
Alexa Fluor 750
FAB666S-100UG
Allophycocyanin
FAB666A
Allophycocyanin/Cy7
FAB666APCCY7
Biotin
FAB666B
CoraFluor 1
FAB666CL1
DyLight 350
FAB666D
DyLight 405
FAB666E
DyLight 488
FAB666K
DyLight 550
FAB666L
DyLight 594
FAB666M
DyLight 650
FAB666W
DyLight 680
FAB666Y
DyLight 755
FAB666Z
Fluorescein
FAB666F
HRP
FAB666H
Janelia Fluor 525
FAB666JF525
Janelia Fluor 549
FAB666I
Janelia Fluor 585
FAB666JF585
Janelia Fluor 635
FAB666JF635
Janelia Fluor 646
FAB666J
Janelia Fluor 669
FAB666JF669
PE/Atto594
FAB666PEATT594
PE/Cy5.5
FAB666PECY55
PE/Cy7
FAB666PECY7
PerCP
FAB666C
Phycoerythrin
FAB666P
mFluor Violet 450 SE
FAB666MFV450
mFluor Violet 500 SE
FAB666MFV500
mFluor Violet 610 SE
FAB666MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB666F
Carrier Free
FAB666P
Carrier Free
FAB666A
Carrier Free
MAB666-SP
Carrier Free
MAB666-500
Carrier Free
FAB666S-100UG
Carrier Free
FAB666R-100UG
Carrier Free
FAB666N-100UG
Carrier Free
FAB666G-100UG
Carrier Free
FAB666V-100UG
Carrier Free
FAB666U-100UG
Carrier Free
FAB666T-100UG
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DNAM-1/CD226 Antibody (102511) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DNAM-1/CD226
Glu19-Asn247 (predicted)
Accession # Q15762
Specificity
Detects human DNAM-1/CD226 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD226
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB666 in the following applications:
Publications
Read Publications using
MAB666 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DNAM-1/CD226 Antibody (102511) [Unconjugated]

  • CD226 antigenplatelet and T cell activation antigen 1
  • CD226 molecule
  • CD226
  • DNAM1
  • DNAM-1
  • DNAM1adhesion glycoprotein
  • DNAM-1DNAX accessory molecule-1
  • DNAX accessory molecule 1
  • PTA1
  • T lineage-specific activation antigen 1 antigen
  • TLiSA1

Background

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).

  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene July 16 epub.
  12. Shibuya, K. et al. (1999) Immunity 11:615.
  13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNAM-1/CD226 Antibody (MAB666)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Neutralization.


Filter By Application
Bioassay
(1)
Flow Cytometry
(4)
Neutralization
(3)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB666 Applications Species
S Sen Santar, DJ Lee,  Crespo, JJ Hu, C Walker, X Ma, Y Zhang, S Chowdhury, KF Meza-Sosa, M Lewandrows, H Zhang, M Rowe, A McClelland, H Wu, C Junqueira, J Lieberman The NK�cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells Nature, 2023-04-05;616(7956):348-356. 2023-04-05 [PMID: 37020026] (Neutralization, Human) Neutralization Human
A Chashchina, M Märklin, C Hinterleit, HR Salih, JS Heitmann, B Klimovich DNAM-1/CD226 is functionally expressed on acute myeloid leukemia (AML) cells and is associated with favorable prognosis Scientific Reports, 2021-09-09;11(1):18012. 2021-09-09 [PMID: 34504191] (Flow Cytometry, Human) Flow Cytometry Human
Elaine Y.L. Leung, Darren P. Ennis, Philippa R. Kennedy, Christopher Hansell, Suzanne Dowson, Malcolm Farquharson et al. NK Cells Augment Oncolytic Adenovirus Cytotoxicity in Ovarian Cancer Molecular Therapy - Oncolytics 2020-03-27 [PMID: 32195317]
DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 6/11/2019 [PMID: 31184599]
M Najar, M Fayyad-Kaz, N Meuleman, D Bron, H Fayyad-Kaz, L Lagneaux Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation J Cell Commun Signal, 2018-01-19;0(0):. 2018-01-19 [PMID: 29350342] (Neutralization, Human) Neutralization Human
S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2017-11-06;7(2):e1392426. 2017-11-06 [PMID: 29308322] (Bioassay, Human) Bioassay Human
JL Espinoza, LQ Trung, PT Inaoka, K Yamada, DT An, S Mizuno, S Nakao, A Takami The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans Oxid Med Cell Longev, 2017-05-04;2017(0):6781872. 2017-05-04 [PMID: 28546852] (Flow Cytometry, Human) Flow Cytometry Human
Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses, 2016-07-20;33(2):93-100. 2016-07-20 [PMID: 27296670] (Neutralization, Human) Neutralization Human
Huijskens M, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers B, Tilanus M, Bos G, Wieten L, Germeraad W Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy. Cytotherapy, 2015-03-05;17(5):613-20. 2015-03-05 [PMID: 25747742] (Flow Cytometry, Human) Flow Cytometry Human
Magnani CF, Alberigo G, Bacchetta R, Serafini G, Andreani M, Roncarolo MG, Gregori S Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells. Eur. J. Immunol., 2011-05-13;41(6):1652-62. 2011-05-13 [PMID: 21469116] (Flow Cytometry, Human) Flow Cytometry Human

Review for DNAM-1/CD226 Antibody (MAB666) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB666:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 10/24/2021
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Summary

ApplicationWestern Blot
Sample TestedMesenchymal stromal cells
SpeciesHuman
Lot102511

Comments

Commentsprimary antibody dilution was 1:1000, secondary 1:4000

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Western Blot DNAM-1/CD226 MAB666
Enlarge
 Verified Customer
10/24/2021
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol CD226
Uniprot