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DLG7/HURP Antibody

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Orthogonal Strategies: Western Blot: DLG7/HURP Antibody [NBP1-87976] - Analysis in human cell lines U2OS and SK-MEL-30 using anti-DLGAP5 antibody. Corresponding DLGAP5 RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: DLG7/HURP Antibody [NBP1-87976] - Staining of human cell line A-431 shows localization to cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Analysis in human testis and liver tissues using NBP1-87976 antibody. Corresponding DLGAP5 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human pancreas shows no positivity in exocrine or endocrine glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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DLG7/HURP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRILVELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLGAP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DLG7/HURP Protein (NBP1-87976PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DLG7/HURP Antibody

  • DAP-5
  • Discs large homolog 7
  • discs, large (Drosophila) homolog-associated protein 5
  • disks large-associated protein 5
  • DLG7
  • Hepatoma up-regulated protein
  • HURPDLG1
  • KIAA0008discs, large homolog 7 (Drosophila)
  • large homolog 7

Background

DLG7/HURP is a novel component of the Ran-importin spindle assembly pathway. DLG7/HURP binds microtubules and affects their organization both in vitro and in vivo. In egg extract, anti-DLG7/HURP antibodies disrupt the formation of both Ran-dependent and chromatin and centrosome-induced spindles. DLG7/HURP is also required for the proper formation and function of mitotic spindles in HeLa cells. DLG7/HURP is also over-expressed in some tumor types

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DLGAP5