Dihydrolipoamide Dehydrogenase/DLD Antibody

Images

 
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown ...read more
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining of human kidney shows high expression.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma ...read more
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining of human epididymis shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining in human kidney and epididymis tissues using anti-DLD antibody. Corresponding DLD RNA-seq ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Dihydrolipoamide Dehydrogenase/DLD Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: DVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVK
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
Dihydrolipoamide Dehydrogenase/DLD Protein (NBP2-13926PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody

  • 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
  • branched chain keto acid dehydrogenase complex
  • Diaphorase
  • dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
  • Dihydrolipoamide Dehydrogenase
  • dihydrolipoamide dehydrogenasemitochondrial
  • DLD
  • DLDD
  • DLDH
  • E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
  • E3
  • EC 1.8.1
  • EC 1.8.1.4
  • GCSL
  • Glycine cleavage system L protein
  • glycine cleavage system protein L
  • LAD
  • Lipoamide Dehydrogenase
  • lipoamide reductase
  • lipoyl dehydrogenase
  • PHE3

Background

Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DLD