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Desmin Antibody (RD301) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, Ha, Rb, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
RD301
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

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Desmin Antibody (RD301) [Janelia Fluor® 646] Summary

Immunogen
Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal extract of Chicken gizzard.
Specificity
This antibody reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DES
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined..

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Desmin Antibody (RD301) [Janelia Fluor® 646]

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle.DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (Debus E et al.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Desmin Antibody (NBP1-97707JF646) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Desmin Antibody (NBP1-97707JF646)

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Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

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Bioinformatics

Gene Symbol DES