Reactivity | Hu, Mu, Rt, Po, Ca, Ch, Ha, Rb, ZeSpecies Glossary |
Applications | WB, ICC/IF, IHC |
Clone | RD301 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1 mg/ml |
Description | The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
Immunogen | Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal extract of Chicken gizzard. |
Specificity | This antibody reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | DES |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This antibody is suitable for immunoblotting, immunocytochemistry on permeabilized cells and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS, pH 7.2 |
Preservative | 0.09% Sodium Azide |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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Gene Symbol | DES |