![Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771] - Western blot of HeLa cell lysate showing specificimmunolabeling of the ~142 kDa DENND3 proteinphosphorylated at Ser554 in the first lane (-). Phosphospecificityis shown in the second lane (+) where immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lambda-Ptase, 800 units/1mg protein for 30 min).](http://images.novusbio.com/fullsize/DENND3-[p-Ser554]-Antibody-Western-Blot-NBP2-60771-img0001.jpg "Western Blot: DENND3 [p Ser554] Antibody [NBP2-60771] - Western blot of HeLa cell lysate showing specificimmunolabeling of the ~142 kDa DENND3 proteinphosphorylated at Ser554 in the first lane (-). Phosphospecificityis shown in the second lane (+) where immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lambda-Ptase, 800 units/1mg protein for 30 min).")
| Reactivity | Hu, Mu, Rt, Pm, ShSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 conjugated to KLH. Accession # D4A2H4 |
| Modification | p Ser554 |
| Specificity | Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with lambda-phosphatase. |
| Predicted Species | Mouse (100%), Rat (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DENND3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 142 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
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![DENND3 [p Ser554] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "DENND3 [p Ser554] Antibody")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DENND3 |
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")