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ETFB Antibody

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Western Blot: ETFB Antibody [NBP1-89545] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: ETFB Antibody [NBP1-89545] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP1-89545] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria & vesicles.
Immunohistochemistry-Paraffin: ETFB Antibody [NBP1-89545] - Staining of human heart muscle shows cytoplasmic positivity with a granular pattern in myocytes.
Independent Antibodies: Western Blot: ETFB Antibody [NBP1-89545] - Analysis using Anti-ETFB antibody NBP1-89545 (A) shows similar pattern to independent antibody NBP1-86040 (B).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

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ETFB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETFB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ETFB Protein (NBP1-89545PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ETFB Antibody

  • beta-ETF
  • electron transfer flavoprotein beta subunit
  • electron transfer flavoprotein beta-subunit
  • electron transfer flavoprotein subunit beta
  • electron transfer flavoprotein, beta polypeptide
  • electron-transfer-flavoprotein, beta polypeptide
  • electron-transferring-flavoprotein, beta polypeptide
  • MADD

Background

ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ETFB