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DBT Antibody - Azide and BSA Free

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Western Blot: DBT Antibody [NBP3-16010] - Western blot analysis of extracts of various cell lines, using DBT antibody (NBP3-16010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: DBT Antibody [NBP3-16010] - Western blot analysis of extracts of various cell lines, using DBT antibody (NBP3-16010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry-Paraffin: DBT Antibody [NBP3-16010] - Immunohistochemistry of paraffin-embedded mouse kidney using DBT Rabbit pAb (NBP3-16010) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-16010-20ul
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DBT Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3). SRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DBT
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for DBT Antibody - Azide and BSA Free

  • BCATE2BCKAD E2 subunit
  • BCKADE2
  • BCKAD-E2
  • branched chain acyltransferase, E2 component
  • Branched-chain alpha-keto acid dehydrogenase complex component E2
  • Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex
  • dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease)
  • dihydrolipoamide branched chain transacylase E2
  • Dihydrolipoamide branched chain transacylase
  • dihydrolipoyl transacylase
  • Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
  • E2 component of branched chain alpha-keto acid dehydrogenase complex
  • E2
  • E2B
  • EC 2.3.1.168
  • lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial
  • lipoamide acyltransferase component of mitochondrial branched-chain alpha-ketoacid dehydrogenase complex
  • MGC9061
  • mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit(E2b)

Background

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DBT Antibody -
Azide and BSA Free
NBP3-16010
DBT Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Product General Protocols

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Bioinformatics

Gene Symbol DBT