![Western Blot: DBT Antibody [NBP1-31760] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DBT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0011.jpg "Western Blot: DBT Antibody [NBP1-31760] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DBT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
| Reactivity | Hu, Mu, Rt, Bv, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DBT. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Predicted Species | Primate (100%), Bovine (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DBT |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for DBT Antibody (NBP1-31760)Find related products by research area.
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| Gene Symbol | DBT |
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-31760] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DBT stained by DBT antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31760-img0009.jpg "Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-31760] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DBT stained by DBT antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-31760] - Mouse kidney. DBT antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-31760-img0004.jpg "Immunohistochemistry-Paraffin: DBT Antibody [NBP1-31760] - Mouse kidney. DBT antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: DBT Antibody [NBP1-31760] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0003.jpg "Western Blot: DBT Antibody [NBP1-31760] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0005.jpg "Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug mouse liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0006.jpg "Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug mouse liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")