![Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.](http://images.novusbio.com/fullsize/DARC-Antibody-2C3-Chimeric-Western-Blot-NBP2-75197-img0001.jpg "Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, B/N |
| Clone | 2C3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | CHO cells transfected with cDNA encoding the Duffy glycoprotein. |
| Specificity | The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ACKR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for DARC Antibody (NBP2-75197)Find related products by research area.
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| Gene Symbol | ACKR1 |
![Immunofluorescence: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (NBP2-75197) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-2C3-Chimeric-Immunofluorescence-NBP2-75197-img0002.jpg "Immunofluorescence: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (NBP2-75197) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
