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D4S234E Overexpression Lysate

Name: D4S234E Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-08500
Price: 679 USD

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Western Blot: D4S234E Overexpression Lysate (Adult Normal) [NBP2-08500] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for D4S234E.

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB

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D4S234E Overexpression Lysate Summary

Description

D4S234E Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC206927

Accession#: NM_001040101

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Gene

NSG1

Applications/Dilutions

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	20.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Array

Type

Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for D4S234E Overexpression Lysate

carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein
D4S234Brain neuron cytoplasmic protein 1
DNA segment on chromosome 4 (unique) 234 expressed sequence
NEEP21
neuron-specific protein family member 1
NSG1
P21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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D4S234E
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Bioinformatics

Gene Symbol

NSG1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

D4S234E Overexpression Lysate

Species Glossary

Images

Product Details

Order Details

D4S234E Overexpression Lysate Summary

D4S234E Transient Overexpression Lysate

Applications/Dilutions

Packaging, Storage & Formulations

Lysate Details for Array

Notes

Alternate Names for D4S234E Overexpression Lysate

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for D4S234E Lysate (NBP2-08500) (0)

Reviews for D4S234E Lysate (NBP2-08500) (0)

Product General Protocols

Video Protocols

FAQs for D4S234E Lysate (NBP2-08500) (0)

Additional D4S234E Products

Research Areas for D4S234E Lysate (NBP2-08500)

Blogs on D4S234E

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Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics