![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Human Lung Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-KRT7-1198-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48145-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Human Lung Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | IHC |
| Clone | KRT7/1198 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729) |
| Localization | Cytoplasmic |
| Marker | Glandular and Transitional Epithelial Marker |
| Specificity | It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 7 Antibody (NBP2-48145)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT7 |
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Rat Lung stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-KRT7-1198-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48145-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Rat Lung stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-KRT7-1198-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48145-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1198) - IHC-Prediluted [NBP2-48145] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)")
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Immunohistochemistry CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM7_AF4478_Immunohistochemistry_20616.jpg)
![Western Blot CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM-7_AF4478_Western_Blot_5475.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")