![Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Western-Blot-NB500-352-img0002.jpg "Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.")
| Reactivity | Hu, Po, Bv, Mu(-), Rb(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | C-46 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Cytoskeleton preparation of HeLa human cervix carcinoma cell line. |
| Specificity | The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT7 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot - Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) Immunohistochemistry-Paraffin sections - Pretreatment: 0,1% pepsin, 30 min, 37C Incubation: 4C overnight Application note: chromogen DAB. This antibody is CyTOF ready. |
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| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
| Publication using NB500-352 | Applications | Species |
|---|---|---|
| Cheng, K S, Pan, R Et al. ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells. Theranostics 2020-10-13 [PMID: 33042268] (IF/IHC, Human) | IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 7/17 Antibody (NB500-352)Find related products by research area.
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![Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Immunohistochemistry-Paraffin-NB500-352-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).")
![Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Intracellular-NB500-352-img0006.jpg "Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Fig. 1. Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7/17 Antibody (C-46).](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Immunohistochemistry-NB500-352-img0001.jpg "Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Fig. 1. Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7/17 Antibody (C-46).")
![Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Cytometry-NB500-352-img0004.jpg "Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on A549 cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Cytometry-NB500-352-img0005.jpg "Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on A549 cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")