![Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-278202416285243.jpg "Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SA30-06 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400. (Uniprot: P08727) |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT19 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Recommended dilution for Multiplex IHC: 1:5000-1:10000 |
| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 19 Antibody (NBP3-32248)Find related products by research area.
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| Gene Symbol | KRT19 |
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414115431.jpg "Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414333689.jpg "Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - ICC staining of Cytokeratin 19 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32248, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-29202410484831.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - ICC staining of Cytokeratin 19 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32248, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Flow Cytometry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32248, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-39202488466.jpg "Flow Cytometry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32248, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).")
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