![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0007.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
| Reactivity | Hu, Ma(-), Rt(-)Species Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | A53-B/A2.26 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15186). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Human breast cancer MCF-7 cells |
| Epitope | aa 312-335 |
| Localization | Cytoplasmic |
| Marker | Pancreatic Stem Cell Marker |
| Specificity | This monoclonal antibody reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. |
| Isotype | IgG2a Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT19 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 19 Antibody (NBP2-33134)Find related products by research area.
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| Gene Symbol | KRT19 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)")
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33134-img0008.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).")
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0006.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.")
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33134-img0001.jpg "Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.")
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0002.jpg "Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0003.jpg "Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.")
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