Novus Biologicals products are now on bio-techne.com

Cytokeratin 19 Antibody (963420) [Unconjugated]

Images

 
Orthogonal Strategies: Cytokeratin 19 mRNA (red) andprotein (green) was detected in formalin-fixed paraffin-embedded tissuesections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog# ...read more
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, ZR-75 human breast cancer cell line, DU145 human prostate carcinoma cell line, MCF-7 human breast cancer cell line, and A431 human epithelial ...read more
Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 8 µg/mL for 3 hours at room temperature. Cells were ...read more
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 5 µg/mL for 1 hour at ...read more
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for 1 hour at ...read more
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human papillary thyroid cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for ...read more
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for 1 hour at room ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cytokeratin 19 at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, Dual ISH-IHC, ICC/IF
Clone
963420
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Cytokeratin 19 Antibody (963420) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human Cytokeratin 19
Gln311-Lys370
Accession # P08727
Specificity
Detects human Cytokeratin 19 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytokeratin 19 Antibody (963420) [Unconjugated]

  • 40-kDa keratin intermediate filament
  • CK19
  • CK-19
  • Cytokeratin 19
  • EndoC
  • K19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Cytokeratin 19 (Keratin, type I cytoskeletal 19; also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat; mammary; bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82% aa sequence identity with mouse Cytokeratin 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-47940
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
NBP2-34594
Species: Hu, Pm
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P
AF4478
Species: Hu
Applications: IHC, WB
NBP3-41306
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP2-67309
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-16094
Species: Hu, Mu, Ze
Applications: ICC/IF, IHC-WhMt, IHC, IHC-P, KO, WB
MAB1368
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, KO, Simple Western, WB
NBP2-34270
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
8184-CK
Species: Hu
Applications: EnzAct
NBP1-85630
Species: Hu
Applications: IHC, IHC-P, WB
NB120-22711
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB7619
Species: Hu
Applications: ICC, IHC, Simple Western, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NB110-58870
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KD, Simple Western, WB
NBP2-61736
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, IHC, IHC-P, WB
1129-ER
Species: Hu
Applications: BA
AF7355
Species: Hu
Applications: ICC, Simple Western, WB

Publications for Cytokeratin 19 Antibody (MAB35061) (0)

There are no publications for Cytokeratin 19 Antibody (MAB35061).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cytokeratin 19 Antibody (MAB35061) (0)

There are no reviews for Cytokeratin 19 Antibody (MAB35061). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cytokeratin 19 Antibody (MAB35061) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 19 Antibody (963420) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Uniprot