![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31326-img0009.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Rt, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary. |
| Localization | Cytoplasm, Nucleus |
| Predicted Species | Mouse (90%), Bovine (96%), Canine (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KRT14 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-FR assay dependent |
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-31326-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-91020242013123.jpg "Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000.")
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-9102024195726.jpg "Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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