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Human Cytochrome P450 1A2 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human Cytochrome P450 1A2  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31330] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.051 ng/mL (example only; lot dependent)

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Human Cytochrome P450 1A2 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.051 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CYP1A2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Cytochrome P450 1A2 - Ready-To-Use ELISA Kit (Colorimetric)

  • aryl hydrocarbon hydroxylase
  • CP12
  • CYP1A2
  • CYPIA2
  • Cytochrome P(3)450
  • Cytochrome P450 1A2
  • Cytochrome P450 4
  • cytochrome P450, family 1, subfamily A, polypeptide 2
  • cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2
  • Cytochrome P450-P3
  • dioxin-inducible P3-450
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • P3-450
  • P450 form 4
  • P450(PA)
  • xenobiotic monooxygenase

Background

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CYP1A2