![Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cy](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Western-Blot-NBP2-70518-img0001.jpg "Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cy")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI8F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYP1A2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 58.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome P450 1A2 Antibody (NBP2-70518)Find related products by research area.
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| Gene Symbol | CYP1A2 |
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70518-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0012.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70518-img0011.jpg "Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0005.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0006.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0007.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0008.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0009.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human lung tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70518-img0010.jpg "Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human lung tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.")
![Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Analysis of Jurkat cells, using anti-Cytochrome P450 1A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70518-img0002.jpg "Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Analysis of Jurkat cells, using anti-Cytochrome P450 1A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cytochrome P450 1A2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A2-Antibody-OTI8F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70518-img0003.jpg "Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cytochrome P450 1A2 antibody, and then analyzed by flow cytometry.")
