Cytochrome P450 1A1 Antibody Summary
Immunogen |
Purified, His-tagged, full length human P450 1A1 fusion protein. |
Specificity |
Detects Cytochrome P450 1A1 from human cells. This detects, to a lesser extent, other P450 isoforms. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CYP1A1 |
Purity |
Ammonium sulfate precipitation |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 2 ug/ml
- Western Blot 1:500
|
Application Notes |
WB: Detects an approx. 54 kDa protein representing P450 1A1 from MCF-7 cell extract. |
Control |
|
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Purity |
Ammonium sulfate precipitation |
Alternate Names for Cytochrome P450 1A1 Antibody
Background
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for Cytochrome P450 1A1 Antibody (NB300-608) (0)
Control Lysate(s)
Secondary Antibodies
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