Cytochrome P450 1A1 Antibody

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Western Blot: Cytochrome P450 1A1 Antibody [NB300-608] - Analysis of cytochrome P450 1A1 from MCF-7 cell extract.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cytochrome P450 1A1 Antibody Summary

Immunogen
Purified, His-tagged, full length human P450 1A1 fusion protein.
Specificity
Detects Cytochrome P450 1A1 from human cells. This detects, to a lesser extent, other P450 isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP1A1
Purity
Ammonium sulfate precipitation
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Western Blot 1:500
Application Notes
WB: Detects an approx. 54 kDa protein representing P450 1A1 from MCF-7 cell extract.
Control
MCF-7 Whole Cell Lysate
Cytochrome P450 1A1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for Cytochrome P450 1A1 Antibody

  • AHH
  • AHRR
  • aryl hydrocarbon hydroxylase
  • CP11
  • CYP1
  • CYP1A1
  • CYPIA1
  • cytochrome P1-450, dioxin-inducible
  • Cytochrome P450 1A1
  • Cytochrome P450 form 6
  • cytochrome P450, family 1, subfamily A, polypeptide 1
  • Cytochrome P450-C
  • Cytochrome P450-P1
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • P1-450
  • P450-C
  • P450DX
  • subfamily I (aromatic compound-inducible), polypeptide 1
  • xenobiotic monooxygenase

Background

The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP1A1
Entrez
Uniprot