Home » Cystatin A » Cystatin A Antibodies » Cystatin A Antibody [Unconjugated]

Cystatin A Antibody [Unconjugated]

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Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1407) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1407
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Catalog# & Formulation Size Price
Carrier Free
AF1407-SP
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Cystatin A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Cystatin A
Ile2-Phe98
Accession # P01040
Specificity
Detects Cystatin A in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Cystatin A, recombinant human (rh) Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin F, rhCystatin S, rhCystatin SA and rhCystatin SN is observed.  
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSTA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using AF1407.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin A Antibody [Unconjugated]

  • AREI
  • CSTA
  • cystatin A (stefin A)
  • Cystatin A
  • cystatin AS
  • cystatin-A
  • Stefin A
  • STF1
  • STF1Cystatin-AS
  • STFA
  • STFAStefin-A

Background

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

  1. Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
  2. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  3. Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
  4. Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
  5. Sinha, A.A. et al. (2002) Cancer 94:3141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSTA
Uniprot