![Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Western-Blot-NBP3-07484-img0004.jpg "Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | CCNE1/2460 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08805) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant human Cyclin E1 protein fragment (around aa 10-176) (exact sequence is proprietary) (Uniprot: IDP24864) |
| Localization | Nuclear |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CCNE1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_E2_MAB7444_Immunohistochemistry_16977.jpg)
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/12177.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin E1 Antibody (NBP3-07484)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CCNE1 |
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-07484-img0003.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).")
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-07484-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).")
![Protein Array: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Protein-Array-NBP3-07484-img0001.jpg "Protein Array: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).")
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Cyclin E1 stained with CODEX in tonsil tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-07484_mouse-monoclonal-cyclin-e1-antibody-ccne1-2460-1772024944231.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Cyclin E1 stained with CODEX in tonsil tissue. Image from verified customer review.")
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
