![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0007.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 3E9Z3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 11-210 of human Cyclin A2 (P20248). TREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSM |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CCNA2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin A2 Antibody (NBP3-15625)Find related products by research area.
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| Gene Symbol | CCNA2 |
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of U-2 OS cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of U-2 OS cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded mouse liver using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0003.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded mouse liver using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0008.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of C6 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of C6 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded rat spleen using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded rat spleen using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Western_Blot_5394.jpg)
![Immunohistochemistry Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Immunohistochemistry_6819.jpg)
![Simple Western Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Simple_Western_20498.jpg)
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg)
![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![Immunohistochemistry NOV/CCN3 Antibody [Unconjugated]](https://images.novusbio.com/images2/NOV_AF1640_Immunohistochemistry_7314.jpg)
![Western Blot Cyclin A1 Antibody (722407) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_A1_MAB7046_Western_Blot_11398.jpg)
![Immunohistochemistry Cyclin A1 Antibody (722407) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_A1_MAB7046_Immunohistochemistry_11411.jpg)
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![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")