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CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

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Fluorophore-linked immunosorbent assay: CXCR1/IL-8 RA Antibody (8F1-1-4) [NBP1-43339] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD181 (CXCR1) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE.
Fluorophore-linked immunosorbent assay: CXCR1/IL-8 RA Antibody (8F1-1-4) [NBP1-43339] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (left) or 0.25 ug of ...read more

Product Details

Summary
Reactivity Hu, HaSpecies Glossary
Applications ELISA, Flow, IHC
Clone
8F1-1-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43339AF350
Alexa Fluor 405
NBP1-43339AF405
Alexa Fluor 488
NBP1-43339AF488
Alexa Fluor 532
NBP1-43339AF532
Alexa Fluor 594
NBP1-43339AF594
Alexa Fluor 647
NBP1-43339AF647
Alexa Fluor 700
NBP1-43339AF700
Alexa Fluor 750
NBP1-43339AF750
Allophycocyanin
NBP1-43339APC
Allophycocyanin/Cy7
NBP1-43339APCCY7
Biotin
NBP1-43339B
CoraFluor 1
NBP1-43339CL1
DyLight 350
NBP1-43339UV
DyLight 405
NBP1-43339V
DyLight 488
NBP1-43339G
DyLight 550
NBP1-43339R
DyLight 594
NBP1-43339DL594
DyLight 650
NBP1-43339C
DyLight 680
NBP1-43339FR
DyLight 755
NBP1-43339IR
FITC
NBP1-43339F
HRP
NBP1-43339H
Janelia Fluor 525
NBP1-43339JF525
Janelia Fluor 549
NBP1-43339JF549
Janelia Fluor 585
NBP1-43339JF585
Janelia Fluor 635
NBP1-43339JF635
Janelia Fluor 646
NBP1-43339JF646
Janelia Fluor 669
NBP1-43339JF669
PE
NBP1-43339PE
PE/Atto594
NBP1-43339PEATT594
PE/Cy5.5
NBP1-43339PECY55
PE/Cy7
NBP1-43339PECY7
PerCP
NBP1-43339PCP
mFluor Violet 450 SE
NBP1-43339MFV450
mFluor Violet 500 SE
NBP1-43339MFV500
mFluor Violet 610 SE
NBP1-43339MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-43339-0.025mg
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CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free Summary

Immunogen
The immunogen for this antibody was CD181.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CXCR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

  • CD128
  • CD181 antigen
  • CD181
  • CDw128aC-C
  • chemokine (C-X-C motif) receptor 1
  • CKR-1
  • CMKAR1
  • C-X-C chemokine receptor type 1
  • CXCR1
  • CXC-R1
  • CXCR-1
  • High affinity interleukin-8 receptor A
  • IL-8 RA
  • IL-8 receptor type 1
  • IL-8R A
  • IL8R1
  • IL8RA
  • IL8RAC-C-CKR-1
  • IL8RBA
  • interleukin 8 receptor, alpha
  • interleukin-8 receptor type 1
  • interleukin-8 receptor type A

Background

The 8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of its ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-43339
CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free
Species: Hu, Ha
Applications: ELISA, Flow, IHC

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Bioinformatics

Gene Symbol CXCR1
Entrez
Uniprot