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CRHR1/CRF1 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1778] - Analysis of anti-CRF1 antibody with rat hypothalamus.
Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1778] - Analysis of anti-CRF1 antibody with human brain, neurons and glia at 13 ug/ml.

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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CRHR1/CRF1 Antibody - BSA Free Summary

Description
Product can be stored undiluted at 4C for up to 1 month.
Immunogen
Synthetic 17 amino acid peptide from N-terminal extracellular domain of human CRHR1/CRF1.
Epitope
N-Terminus extracellular domain
Localization
Secreted
Specificity
Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRHR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 13 ug/ml
  • Immunohistochemistry-Paraffin 13 ug/ml
  • Knockdown Validated
  • Western Blot
Application Notes
Use in ICC/IF reported in scientific literature (PMID : 29082104). Knockdown validation (PMID: 31881185). Use in Western Blot reported in scientific literature (PMID:31881185)..
Publications
Read Publications using
NLS1778 in the following applications:

Reactivity Notes

Summary of Expression: CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries. Predicted cross-reactivity based on sequence identity: Marmoset (94%), Rat (82%).

Packaging, Storage & Formulations

Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody - BSA Free

  • CHR-R1
  • corticotropin releasing hormone receptor 1
  • corticotropin-releasing factor receptor 1
  • corticotropin-releasing factor type 1 receptor
  • Corticotropin-releasing hormone receptor 1
  • CRF1
  • CRFR
  • CRF-R
  • CRFR1
  • CRF-R1
  • CRF-R-1
  • CRFR-1
  • CRHR1
  • CRH-R-1
  • CRHR1f
  • CRH-R1h
  • CRHR1L
  • CRHRCRH-R1
  • seven transmembrane helix receptor

Background

Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing. CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRHR1/CRF1 Antibody (NLS1778)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, KD, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
KD
(1)
WB
(1)
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Filter By Species
Human
(1)
Rat
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRHR1/CRF1 Antibody (NLS1778). (Showing 1 - 1 of 1 FAQs).

  1. He wonders whether it is suitable for mouse frozen section by IF. Can you so kind to help him and me?
    • Our CRF1 antibody with catalogue number NLS1778 has been validated for use with samples from mouse, however it has only currently been tested for immunohistochemistry with paraffin-embedded tissue, and for immunocytochemistry. It has not yet been tested for immunohistochemistry with frozen tissue. Please refer your customer to our Innovator's Reward if they wish to try using this antibody for IHC with their frozen mouse tissue samples.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRHR1
Entrez
Uniprot