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CRHBP Antibody [Unconjugated]

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CRHBP was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Goat Anti-Human CRHBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2796) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
CRHBP was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Goat Anti-Human CRHBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2796) at 15 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2796
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2796-SP
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CRHBP Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRHBP
Tyr25-Leu322
Accession # P24387.2
Specificity
Detects human CRHBP in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse CRHBP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRHBP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2796 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRHBP Antibody [Unconjugated]

  • corticotropin releasing hormone binding protein
  • corticotropin-releasing factor-binding protein
  • Corticotropin-releasing hormone-binding protein
  • CRFBP
  • CRFBPcorticotropin releasing hormone-binding protein
  • CRF-BPCRF-binding protein
  • CRHBP
  • CRH-BP

Background

Human CRHBP is a 37 kDa secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41 kDa complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRHBP Antibody (AF2796)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Western Blot.


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(2)
Western Blot
(1)
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(2)
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Product General Protocols

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Bioinformatics

Gene Symbol CRHBP
Uniprot