![Western Blot: Urocortin 3 Antibody [NB100-93444] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DYKDDDDK tag probed with NB100-93444 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with NB100-93444 (1mg/ml) in Lane B.](http://images.novusbio.com/fullsize/Urocortin-3-Antibody-Western-Blot-NB100-93444-img0003.jpg "Western Blot: Urocortin 3 Antibody [NB100-93444] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DYKDDDDK tag probed with NB100-93444 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with NB100-93444 (1mg/ml) in Lane B.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KPRQDTAKSPHRTK corresponding to internal region according to NP_444277.2. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | UCN3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of approx. 19 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-93444 | Applications | Species |
|---|---|---|
| Jiang Z, Michal JJ, Williams GA et al. Cross species association examination of UCN3 and CRHR2 as potential pharmacological targets for antiobesity drugs. PLoS ONE 2006-12-20 [PMID: 17183713] |
Secondary Antibodies |
Isotype Controls |
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