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CRHR1/CRF1 Antibody

Images

 
Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by ...read more
Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed ...read more
Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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CRHR1/CRF1 Antibody Summary

Immunogen
Peptide with sequence C-NEEKKSKVHYH corresponding to internal region according to NP_004373.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRHR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). Primary incubation was 1 hour. IHC reported in scientific literature (PMID: 22570383). Use in ICC/IF reported in scientific literature (PMID: 30439403).
Publications
Read Publications using
NBP1-00175 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody

  • CHR-R1
  • corticotropin releasing hormone receptor 1
  • corticotropin-releasing factor receptor 1
  • corticotropin-releasing factor type 1 receptor
  • Corticotropin-releasing hormone receptor 1
  • CRF1
  • CRFR
  • CRF-R
  • CRFR1
  • CRF-R1
  • CRF-R-1
  • CRFR-1
  • CRHR1
  • CRH-R-1
  • CRHR1f
  • CRH-R1h
  • CRHR1L
  • CRHRCRH-R1
  • seven transmembrane helix receptor

Background

The corticotropin-releasing hormone receptor binds to corticotropin-releasing hormone (MIM 122560), a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRHR1/CRF1 Antibody (NBP1-00175)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, Simple Western, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
Simple Western
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(3)
Rat
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRHR1/CRF1 Antibody (NBP1-00175) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRHR1
Entrez
Uniprot