![Western Blot: CPSF6 Antibody [NB100-61596] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiCPSF68 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CPSF6-Antibody-Western-Blot-NB100-61596-img0006.jpg "Western Blot: CPSF6 Antibody [NB100-61596] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiCPSF68 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | Hu, Mu, Bv, Ch, Pm, Xp, ZeSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cleavage and polyadenylation specific factor 6, 68kDa using the numbering given in entry NP_008938.1 (GeneID 11052). |
| Predicted Species | Chicken (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CPSF6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in ICC/IF was reported in the scientific literature (PMID: 23622145). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunocytochemistry__Immunofluorescence_23325.jpg)
![Neutralization Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Block_Neutralize_8975.jpg)
![Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunohistochemistry_9333.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CPSF6 Antibody [NB100-61596] - TNPO3 K.D. and shRNA control HeLa cells were transiently transfected using constructs expressing FLAG-tagged CPSF6. Twenty-four hours post-transfection cells were fixed and immunostained using anti-FLAG antibodies. The nuclear compartment was labeled using DAPI.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23622145/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CPSF6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-61596-img0008.jpg "Immunocytochemistry/Immunofluorescence: CPSF6 Antibody [NB100-61596] - TNPO3 K.D. and shRNA control HeLa cells were transiently transfected using constructs expressing FLAG-tagged CPSF6. Twenty-four hours post-transfection cells were fixed and immunostained using anti-FLAG antibodies. The nuclear compartment was labeled using DAPI.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23622145/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CPSF6-Antibody-Immunohistochemistry-Paraffin-NB100-61596-img0005.jpg "Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CPSF6-Antibody-Immunohistochemistry-Paraffin-NB100-61596-img0004.jpg "Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Simple Western: CPSF6 Antibody [NB100-61596] - Simple Western lane view shows a specific band for CPSF6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CPSF6-Antibody-Simple-Western-NB100-61596-img0003.jpg "Simple Western: CPSF6 Antibody [NB100-61596] - Simple Western lane view shows a specific band for CPSF6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunoprecipitation: CPSF6 Antibody [NB100-61596] - Detection of human CPSF68 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF68 antibody NB100-61596 (lot NB100-61596-2) used for IP at 3 ug per reaction. CPSF68 was also immunoprecipitated by a previous lot of this antibody (lot NB100-61596-1) and another rabbit anti-CPSF68 antibody. For blotting immunoprecipitated CPSF68, NB100-61596 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CPSF6-Antibody-Immunoprecipitation-NB100-61596-img0007.jpg "Immunoprecipitation: CPSF6 Antibody [NB100-61596] - Detection of human CPSF68 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF68 antibody NB100-61596 (lot NB100-61596-2) used for IP at 3 ug per reaction. CPSF68 was also immunoprecipitated by a previous lot of this antibody (lot NB100-61596-1) and another rabbit anti-CPSF68 antibody. For blotting immunoprecipitated CPSF68, NB100-61596 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NB100-61596] - Depletion of TNPO3 does not change the localization of CPSF6. (A) TNPO3 K.D. & shRNA control HeLa cells were fixed & stained using specific antibodies against CPSF6 (red), ASF/SF2 (red) & TNPO3 (green), as described in Methods. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 1. (B) TNPO3 K.D. & shRNA control HeLa cells were transiently transfected using constructs expressing FLAG-tagged CPSF6 or ASF/SF2. Twenty-four hours post-transfection cells were fixed & immunostained using anti-FLAG antibodies. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 2. Similar results were obtained in three independent experiments & a representative experiment is shown. Image collected & cropped by CiteAb from the following publication (//retrovirology.biomedcentral.com/articles/10.1186/1742-4690-10-46), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61596_rabbit-polyclonal-cpsf6-antibody-31020241554199.jpg "Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NB100-61596] - Depletion of TNPO3 does not change the localization of CPSF6. (A) TNPO3 K.D. & shRNA control HeLa cells were fixed & stained using specific antibodies against CPSF6 (red), ASF/SF2 (red) & TNPO3 (green), as described in Methods. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 1. (B) TNPO3 K.D. & shRNA control HeLa cells were transiently transfected using constructs expressing FLAG-tagged CPSF6 or ASF/SF2. Twenty-four hours post-transfection cells were fixed & immunostained using anti-FLAG antibodies. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 2. Similar results were obtained in three independent experiments & a representative experiment is shown. Image collected & cropped by CiteAb from the following publication (//retrovirology.biomedcentral.com/articles/10.1186/1742-4690-10-46), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")