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NUDT21 Antibody (OTI13H1)

Images

 
Western Blot: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUDT21.
Immunohistochemistry-Paraffin: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Staining of HT-29 cells using anti-NUDT21 mouse monoclonal antibody (1:100).
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NUDT21 Antibody (OTI13H1) [NBP2-46276] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI13H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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NUDT21 Antibody (OTI13H1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 2-227 of human NUDT21(NP_008937) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NUDT21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NUDT21 Antibody (OTI13H1)

  • CFIm25
  • CFIM25CPSF 25 kDa subunit
  • cleavage and polyadenylation specific factor 5, 25 kD subunit
  • cleavage and polyadenylation specific factor 5, 25 kDa
  • Cleavage and polyadenylation specificity factor 25 kDa subunit
  • cleavage and polyadenylation specificity factor subunit 5
  • CPSF25
  • CPSF5DKFZp686H1588
  • Nucleoside diphosphate-linked moiety X motif 21
  • nudix (nucleoside diphosphate linked moiety X)-type motif 21
  • Nudix motif 21
  • pre-mRNA cleavage factor Im (25kD)
  • Pre-mRNA cleavage factor Im 25 kDa subunit
  • pre-mRNA cleavage factor Im, 25kD subunit

Background

NUDT21 is encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NUDT21