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Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

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ELISA: Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06884] - Samples were spiked with high concentrations of Rat Corticotropin Releasing Factor and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06884] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, ELISA(Comp)
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Corticotropin Releasing Factor in samples. No significant cross-reactivity or interference between Rat Corticotropin Releasing Factor and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
5.89
Intra-Assay
6.06
Spike Recovery
84-99 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CRH

Applications/Dilutions

Dilutions
  • Competitive ELISA
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

  • corticoliberin
  • corticotropin releasing hormone
  • Corticotropin-releasing hormone
  • CRFCorticotropin-releasing factor

Background

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CRH