![Immunocytochemistry/Immunofluorescence: Complexin-2 Antibody (021) [NBP2-90019] - Staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/Complexin-2-Antibody-021-Immunocytochemistry-Immunofluorescence-NBP2-90019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Complexin-2 Antibody (021) [NBP2-90019] - Staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF |
| Clone | 021 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human Complexin-2 (Uniprot#: Q6PUV4-1; Asp2-Lys134). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CPLX2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Complexin-2 Antibody (NBP2-90019)Find related products by research area.
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| Gene Symbol | CPLX2 |
![Flow Cytometry: Complexin-2 Antibody (021) [NBP2-90019] - Analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated, stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Complexin-2-Antibody-021-Flow-Cytometry-NBP2-90019-img0002.jpg "Flow Cytometry: Complexin-2 Antibody (021) [NBP2-90019] - Analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated, stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")