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Complement C3d Antibody (053A-1149.3.1.4 (1003)) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
053A-1149.3.1.4 (1003)
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65510AF350
Alexa Fluor 532
NB100-65510AF532
Alexa Fluor 594
NB100-65510AF594
Alexa Fluor 750
NB100-65510AF750
Allophycocyanin
NB100-65510APC-0.1ml
Biotin
NB100-65510B
DyLight 350
NB100-65510UV-0.1ml
DyLight 405
NB100-65510V-0.1ml
DyLight 550
NB100-65510R
DyLight 594
NB100-65510DL594
DyLight 650
NB100-65510C
DyLight 680
NB100-65510FR-0.1ml
DyLight 755
NB100-65510IR-0.1ml
FITC
NB100-65510F
HRP
NB100-65510H
Janelia Fluor 549
NB100-65510JF549
Janelia Fluor 646
NB100-65510JF646
PE
NB100-65510PE-0.1ml
PerCP
NB100-65510PCP-0.1ml
Unconjugated
NB100-65510-0.1ml
mFluor Violet 450 SE
NB100-65510MFV450
mFluor Violet 500 SE
NB100-65510MFV500
mFluor Violet 610 SE
NB100-65510MFV610
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Complement C3d Antibody (053A-1149.3.1.4 (1003)) [DyLight 488] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Purified C3d from human plasma
Specificity
NB100-65510 is specific for human complement component 3d (C3d), a 33kDa polypeptide fragment generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d. C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Complement C3d Antibody (053A-1149.3.1.4 (1003)) [DyLight 488]

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Background

C3d is a fragment of the major complement protein C3, C3d is formed as a result of the deactivation of C3b.Factor I in the presence of cofactors cleaves C3b to form iC3b and C3f, Factor I and/or other proteasesthen cleaves iC3b to form C3c and C3dg. C3dg is then further cleaved by tryptic enzymes to C3g andC3d. The C3 fragments are then eliminated from circulation by phagocytic cells in the liver. C3d has amolecular weight of 34kDa. Normally C3d is present in trace amounts in plasma, however, in diseasestates where rapid complement consumption is occurring levels of C3d can be elevated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Complement C3d Antibody (053A-1149.3.1.4 (1003)) [DyLight 488]
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Applications: WB, ELISA, Flow, IHC

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol C3