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Clusterin/APOJ Antibody (Hs-3) [FITC]

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Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Fe(-), Po(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
Hs-3
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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Clusterin/APOJ Antibody (Hs-3) [FITC] Summary

Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
Apolipoprotein J (Hs-3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLU
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Clusterin/APOJ Antibody (Hs-3) [FITC]

  • 40
  • 40, sulfated glycoprotein 2
  • Aging-associated gene 4 protein
  • aging-associated protein 4
  • APOJ
  • apo-J
  • Apolipoprotein J
  • CLI
  • CLIclusterin (complement lysis inhibitor, SP-40
  • CLU
  • Clusterin
  • Complement cytolysis inhibitor
  • complement lysis inhibitor
  • Complement-associated protein SP-40
  • Ku70-binding protein 1
  • KUB1SGP2
  • MGC24903
  • NA1/NA2
  • SGP-2
  • SP-40
  • sulfated glycoprotein 2
  • Testosterone-repressed prostate message 2
  • testosterone-repressed prostate message 2, apolipoprotein J)
  • TRPM-2
  • TRPM-2TRPM2

Background

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CLU