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Rat Claudin-5 ELISA Kit (Colorimetric)

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ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Rat Claudin-5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CLDN5 in samples. No significant cross-reactivity or interference between Rat CLDN5 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.28%
Intra-Assay
CV% < 5.66%
Spike Recovery
86-100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CLDN5

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using
NBP2-75333 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Claudin-5 ELISA Kit (Colorimetric)

  • AWAL
  • AWALTransmembrane protein deleted in VCFS
  • BEC1
  • claudin 5
  • Claudin5
  • Claudin-5
  • CLDN5
  • CPETRL1
  • TMDVCF
  • TMVCF1
  • TMVCFTMDVCF
  • transmembrane protein deleted in velocardiofacial syndrome

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Claudin-5 ELISA Kit (NBP2-75333)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ELISA.


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Product PDFs

  SDS
  Rat Claudin-5 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol CLDN5