![Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CKAP4-p63-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15908-img0006.jpg "Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Fluoroshield with DAPI.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CKAP4/p63. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CKAP4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IP- Assay dependent. |
| Theoretical MW | 66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - Human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CKAP4-p63-Antibody-Immunohistochemistry-Paraffin-NBP2-15908-img0005.jpg "Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - Human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/CKAP4-Antibody-Immunohistochemistry-Paraffin-NBP2-15908-img0002.jpg "Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15908_rabbit-polyclonal-ckap4-p63-antibody-910202420122040.jpg "Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CKAP4/p63 antibody [N3C3] detects CKAP4/p63 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.CKAP4/p63 stained by CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15908_rabbit-polyclonal-ckap4-p63-antibody-9102024200101.jpg "Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CKAP4/p63 antibody [N3C3] detects CKAP4/p63 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.CKAP4/p63 stained by CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15908_rabbit-polyclonal-ckap4-p63-antibody-910202420122010.jpg "Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")