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Human Chromogranin A ELISA Kit (Colorimetric)

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ELISA: Human Chromogranin A ELISA Kit (Colorimetric) [NBP2-67242] - Samples were spiked with high concentrations of Human Chromogranin A and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Chromogranin A ELISA Kit (Colorimetric) [NBP2-67242] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL

Order Details

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Human Chromogranin A ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CHGA in samples. No significant cross-reactivity or interference between Human CHGA and analogues was observed.
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.27%
Intra-Assay
CV% < 5.81%
Spike Recovery
87-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CHGA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Chromogranin A ELISA Kit (Colorimetric)

  • betagranin (N-terminal fragment of chromogranin A)
  • Betagranin
  • Catestatin
  • CGA
  • CHGA
  • Chromofungin
  • chromogranin A (parathyroid secretory protein 1)
  • Chromogranin A
  • chromogranin-A
  • Pancreastatin
  • Parastatin
  • Parathyroid Secretory Protein 1
  • Pituitary Secretory Protein I
  • SP-I

Background

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Chromogranin A ELISA Kit (NBP2-67242). (Showing 1 - 1 of 1 FAQ).

  1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
    • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

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Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

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Bioinformatics

Gene Symbol CHGA