![Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10+PHE5+CGA414)](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-LK2H10-+-PHE5-+-CGA-414-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34674-img0003.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10+PHE5+CGA414)")
| Reactivity | Hu, Mu, Rt, Po, PmSpecies Glossary |
| Applications | IHC |
| Clone | LK2H10 + PHE5 + CGA/414 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34240). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Human pheochromocytoma (LK2H10 & PHE5) & recombinant human chromogranin A protein (CGA414) (Uniprot: P10645) |
| Localization | Finely granular cytoplasmic |
| Marker | Neuroendocrine Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHGA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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| Publications using NBP2-34674 | Applications | Species |
|---|---|---|
| Wright JJ, Eskaros A, Windon A et al. Exocrine pancreas in type 1 and type 2 diabetes: different patterns of fibrosis, metaplasia, angiopathy, and adiposity Diabetes 2023-10-26 [PMID: 37881846] | ||
| Bjorklund F Subcellular mapping of cell types in healthy human pancreatic islets Thesis 2021-01-01 (IF/IHC) | IF/IHC |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
Immunofluorescence - Paraffin | Human | 09/23/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Chromogranin A Antibody (NBP2-34674)Find related products by research area.
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Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post. |
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| Verified Customer 09/23/2018 |
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| Application: | Immunofluorescence - Paraffin | |
| Species: | Human |
| Gene Symbol | CHGA |
![Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-LK2H10-+-PHE5-+-CGA-414-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34674-img0001.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10=PHE5+CGA414)](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-LK2H10-+-PHE5-+-CGA-414-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34674-img0002.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10=PHE5+CGA414)")
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